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aav packaging plasmid pucmini icap php eb  (Addgene inc)


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    Addgene inc aav packaging plasmid pucmini icap php eb
    Aav Packaging Plasmid Pucmini Icap Php Eb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+packaging+plasmid/pUCmini-iCAP-PHP%2EeB+(Plasmid+%23103005)/bio_rxiv__64898__2026__01__26__701845-289-1-8
    Average 97 stars, based on 199 article reviews
    aav packaging plasmid pucmini icap php eb - by Bioz Stars, 2026-09
    97/100 stars

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    Bioprocessing:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Plasmid Preparation:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Construct:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Ligation:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Cloning:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Clone Assay:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Expressing:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Polymerase Chain Reaction:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..

    Amplification:

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (Swiech et al., 2015) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, Figure 5—figure supplement 1A). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH- tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-Grid1). ..

    Article Title: Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 ( ) (pX551) expressed from the Mecp2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV- Grid1 ). ..

    Article Title: Delta glutamate receptor conductance drives excitation of dorsal raphe neurons
    Article Snippet: The AAV vector plasmid encoding SpCas9 (pX551) expressed from the MeCP2 promoter was a gift from Feng Zhang (Addgene plasmid # 60957, AAV-Cas9). .. The AAV packaging plasmid encoding a nuclear envelope-embedded eGFP reporter (Addgene 131682) was constructed by amplifying the KASH domain from (Addgene 60231, a gift of Feng Zhang) and fusing it (in-frame) to the end of coding region for eGFP in (Addgene 60058, pOTTC407) using ligation-independent cloning (AAV-empty, ). gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1). ..



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    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers <t>against</t> <t>AAV-DJ</t> capsid proteins and the expressed P2C5-Fc antibody.
    Aav Packaging Plasmid Pmj114 Addgene Rrid Addgene 85995 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    plasmid package paav poxt hm3dq mcherry wpre  (Addgene inc)
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    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers <t>against</t> <t>AAV-DJ</t> capsid proteins and the expressed P2C5-Fc antibody.
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    aav serotype 2 9 packaging plasmids  (Addgene inc)
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    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers <t>against</t> <t>AAV-DJ</t> capsid proteins and the expressed P2C5-Fc antibody.
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    aav serotype sch9 packaging plasmid 79  (Addgene inc)
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    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers <t>against</t> <t>AAV-DJ</t> capsid proteins and the expressed P2C5-Fc antibody.
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    Image Search Results


    (A) Primary cortical neurons were used to test our CreERT2 (Addgene: #210415) and DIO-mCherry constructs delivered using AAV transductions. (B) AAV DIO-mCherry produces minimal Cre-independent mCherry expression in PCNs. (C) Co-delivery of AAV-iCre (Addgene: #210416) and AAV DIO-mCherry to PCNs induces robust mCherry expression in PCNs. (D) AAV-CreERT2 and AAV DIO-mCherry co-transduction induces minimal tamoxifen-independent mCherry expression in PCNs, while addition of 4-hydroxytamoxifen (1µM) following the same AAV-transduction generates robust mCherry expression. (Scale Bar: 200 µm).

    Journal: bioRxiv

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1101/2025.07.17.665447

    Figure Lengend Snippet: (A) Primary cortical neurons were used to test our CreERT2 (Addgene: #210415) and DIO-mCherry constructs delivered using AAV transductions. (B) AAV DIO-mCherry produces minimal Cre-independent mCherry expression in PCNs. (C) Co-delivery of AAV-iCre (Addgene: #210416) and AAV DIO-mCherry to PCNs induces robust mCherry expression in PCNs. (D) AAV-CreERT2 and AAV DIO-mCherry co-transduction induces minimal tamoxifen-independent mCherry expression in PCNs, while addition of 4-hydroxytamoxifen (1µM) following the same AAV-transduction generates robust mCherry expression. (Scale Bar: 200 µm).

    Article Snippet: The coding region for CreERT2 was amplified from MSCV CreERT2 puro (a gift from Tyler Jacks; Addgene; #22776) and inserted into an AAV packaging plasmid flanked by the EF1α promoter and the poly-adenylation signal from bovine growth hormone using ligation-independent cloning (In-Fusion, Clontech).

    Techniques: Construct, Expressing, Transduction

    (A) The DIO-mCherry rat uses human EF1α promoter to drive expression of mCherry fluorescent protein along with the woodchuck post transcriptional regulatory element (WPRE) and the human growth hormone polyadenylation signal (hGHpA). (A) The primer positions for a PCR genotyping assay spanning the 5’ junction of the mCherry cassette is noted by the black arrows and the positions of the primers (blue) and probe (green arrow) for a qPCR-based genotyping assay are also noted. ( B ) Copy number for WT, hemizygous (Hem), and homozygous (Hom) animals. The ratio is transgene copy number to the genomic gene (Ggt1) in genomic DNA samples isolated from the pups of two separate Hem × Hem crosses. The individual ratios of templates (DIO-mCherry/Ggt1) fall into three quanta (0, 0.5, and 1.0). (C) Amplicons (640 bp) produced by the 5’ junction PCR identify wild type (no bands) or carriers hemizygous and homozygous. (D) Images from the striatum of hemizygous DIO-mCherry rats injected with an AAV virus expressing Cre recombinase after two weeks. Low magnification (top row; scale bar: 500 μm) and higher magnification (bottom row; scale bar: 50 μm). Cre immunoreactivity (white) and mCherry epifluorescence (red) colocalize in both sets of images. Overlap Coefficient (OC)=0.90.

    Journal: bioRxiv

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1101/2025.07.17.665447

    Figure Lengend Snippet: (A) The DIO-mCherry rat uses human EF1α promoter to drive expression of mCherry fluorescent protein along with the woodchuck post transcriptional regulatory element (WPRE) and the human growth hormone polyadenylation signal (hGHpA). (A) The primer positions for a PCR genotyping assay spanning the 5’ junction of the mCherry cassette is noted by the black arrows and the positions of the primers (blue) and probe (green arrow) for a qPCR-based genotyping assay are also noted. ( B ) Copy number for WT, hemizygous (Hem), and homozygous (Hom) animals. The ratio is transgene copy number to the genomic gene (Ggt1) in genomic DNA samples isolated from the pups of two separate Hem × Hem crosses. The individual ratios of templates (DIO-mCherry/Ggt1) fall into three quanta (0, 0.5, and 1.0). (C) Amplicons (640 bp) produced by the 5’ junction PCR identify wild type (no bands) or carriers hemizygous and homozygous. (D) Images from the striatum of hemizygous DIO-mCherry rats injected with an AAV virus expressing Cre recombinase after two weeks. Low magnification (top row; scale bar: 500 μm) and higher magnification (bottom row; scale bar: 50 μm). Cre immunoreactivity (white) and mCherry epifluorescence (red) colocalize in both sets of images. Overlap Coefficient (OC)=0.90.

    Article Snippet: The coding region for CreERT2 was amplified from MSCV CreERT2 puro (a gift from Tyler Jacks; Addgene; #22776) and inserted into an AAV packaging plasmid flanked by the EF1α promoter and the poly-adenylation signal from bovine growth hormone using ligation-independent cloning (In-Fusion, Clontech).

    Techniques: Expressing, Genotyping Assay, Isolation, Produced, Injection, Virus

    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers against AAV-DJ capsid proteins and the expressed P2C5-Fc antibody.

    Journal: Frontiers in Immunology

    Article Title: Long-term protective potency of AAV vector-based SARS-CoV-2 prophylaxis in mice and non-human primates

    doi: 10.3389/fimmu.2026.1829230

    Figure Lengend Snippet: Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers against AAV-DJ capsid proteins and the expressed P2C5-Fc antibody.

    Article Snippet: The rAAV-P2C5-Fc vector construct was produced using a three-plasmid AAV-DJ packaging system (Cell Biolabs, USA) by transient transfection of HEK293 cells (obtained from the cell collection of the N.F.

    Techniques: Concentration Assay, Activity Assay, Immunopeptidomics